Thermo Fisher Scientific Alix Recombinant Rabbit Monoclonal Antibody (JM85-31)
Recombinant rabbit monoclonal antibody targeting ALIX for WB, IHC, and Flow applications. Offers high specificity, sensitivity, and lot-to-lot consistency. Suitable for human, mouse, and rat samples. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1:1,000 | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM85-31 |
| Immunogen | Recombinant protein within Human ALIX (aa 1–160) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810050 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
Apoptotic linked-gene-product 2 (ALG-2) interacting protein X (ALIX) is a conserved adaptor protein ubiquitously expressed in cells.
Originally associated with apoptosis, ALIX is now known to participate in multiple cellular processes including:
- Endosomal sorting
- Endocytosis
- Viral budding
- Actin cytoskeleton assembly
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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