Thermo Fisher Scientific Kininogen Light Chain Monoclonal Antibody (288424)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

288424

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Kininogen Glu21-Ser661 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Kininogen Light Chain,uniProtId:O08677-1,ncbiNodeId:10090,antigenRange:21-661,antigenLength:661,antigenImageFileName:MA5-23989_Kininogen_Light_Chain_O08677-1_Rat.svg,antigenImageFileNamePDP:MA5-23989_Kininogen_Light_Chain_O08677-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610487

Product Specific Information

In Western blots, reacts with full length Kininogen (aa 21-661) and several forms of the light chain starting at residue 389, and does not react with the heavy chain. In Western blots, no cross-reactivity with recombinant mouse (rm) Cystatin A, B, C, E/M, recombinant human (rh) Cystatin D, F, S, SA, SN, rmCystatin/Stefin Homolog, rmFetuin A, rhFetuin B, rmHPRG, rhKininogen, or rhKininostatin is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.