Thermo Fisher Scientific SUMO-tag Monoclonal Antibody (4G11E9)
SUMO-tag 단백질 검출용 마우스 단클론 항체로, Western blot 및 ELISA에 적합합니다. SUMO 프로테아제에 의해 쉽게 절단되는 SUMO 태그 단백질을 인식합니다. 고순도 Protein A 정제, lyophilized 형태로 제공되며 연구용으로 사용됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | - |
| ELISA | 0.05–0.2 µg/mL | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4G11E9 |
| Immunogen | Purified recombinant yeast SUMO protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
SUMO tags are emerging as a viable alternative for increasing both the expression and solubility of otherwise hard-to-express proteins. The SUMO tag can be cleanly excised using SUMO protease, which recognizes the conformation of SUMO protein rather than a specific sequence within SUMO.
The SUMO system can be used effectively in both prokaryotic and eukaryotic systems.
GenScript SUMO-tag Antibody (4G11E9), mAb, Mouse is suitable for detecting fusion proteins that contain a SUMO-tag.
This antibody is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with recombinant yeast SUMO protein.
Target Information
SUMO (Small Ubiquitin-like Modifier) is a 100 amino acid sequence necessary for regulation of protein transport and for controlling transcription in eukaryotic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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