Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), PE-Cyanine7

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Dog, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HI30

Immunogen

CD45 antigen

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

View additional formats

• Alexa Fluor 405
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-Alexa Fluor 750
• Biotin
• FITC
• Pacific Blue
• Pacific Orange
• PE
• PE-Alexa Fluor 610
• PE-Cyanine5.5
• PE-Texas Red
• PerCP
• Qdot 605
• Qdot 655
• Qdot 800
• TRI-COLOR
• Request custom conjugation

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with BSA, sucrose

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10372215

Product Specific Information

The R-phycoerythrin (PE)-Cy7 tandem conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a 488 nm laser without occupying traditional channels associated with FITC and PE. Alternatively, the PE dye tandem can be excited using a 532 nm or 561 nm laser.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Analyte Specific Reagent