Thermo Fisher Scientific SNAI2 Monoclonal Antibody (OTI2B11), TrueMAB
SNAI2(SLUG) 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC, ICC, Flow cytometry에 사용 가능. E.coli에서 발현된 full-length human SNAI2 항원으로 제작. Lyophilized 형태, PBS buffer, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B11 |
| Immunogen | Full length human recombinant protein of human SNAI2 produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Slug (SNAI2) is a member of the Snail family of C2H2-type zinc finger transcription factors. It plays a key role in epithelial-mesenchymal transition (EMT) and neural tube formation during vertebrate embryogenesis.
Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-beta. Once expressed, it binds E-box regions of promoters and represses transcription of genes including E-cadherin and Claudin-1.
Overexpression of Slug has been correlated with poor prognosis in breast, esophageal, and colorectal carcinomas. Additionally, Slug can protect hematopoietic progenitor cells from radiation-induced apoptosis by repressing p53-mediated transcription of Puma.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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