Applied Biosystems™

TaqMan™ RNA-to-CT™ 1-Step Kit

The Applied Biosystems TaqMan RNA-to-CT 1-Step Kit delivers consistent RNA target quantification for a wide variety of assays and is자세히 알아보기

The Applied Biosystems TaqMan RNA-to-CT 1-Step Kit delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems comprehensive library of TaqMan Gene Expression assays. The kit is recommended for a variety of applications including general gene expression studies, biomarker discovery, and microarray validation.

Features of the TaqMan RNA-to-CT 1-Step Kit include:
• Accurate across a wide dynamic range for dependable performance
• Validated with TaqMan Gene Expression Assays for easy experimental setup
• Consistent performance with a wide variety of targets, including AT-rich, GC-rich and long sequences
• 1-Step format means fewer pipetting steps leading to less chances for errors

Efficiency across a broad range of input concentrations
Maximum flexibility in RNA template input quantity requires optimal real-time PCR efficiency across a broad range of template quantities. Figure 1 shows the excellent amplification efficiency across six orders of magnitude. The result is comparable to that of the TaqMan RNA-to-CT 2-Step Kit, and is a significant improvement in dynamic range and sensitivity over the TaqMan One-Step RT-PCR Master Mix Reagents.

High sensitivity at low target concentration
The sensitivity of TaqMan RNA-to-CT 1-Step Kit was validated using a synthetic RNA template for which copy number is precisely known. Significant sampling error occurs when measuring low quantities of target, so statistical analysis is required for proper evaluation using multiple replicates. Figure 2 shows the expected quantity of target and corresponding mean CT values. Statistical analysis indicates high confidence of sample quantification based on a T-test (Table I), consistent with as few as 10 copies of target*.

*Results are dependent on a variety of factors, including assay design.

사양

검출 방법Primer-probe

PCR 방법1-step RT-qPCR

중합효소AmpliTaq Gold DNA Polymerase

반응 속도Standard

용도(장비)7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode

패시브 참조 염료ROX (Pre-mixed)

제품라인RNA-to-CT™, TaqMan™

제품 유형One-Step RT-PCR Kit

순도 또는 품질 등급UP (Ultra Pure)

수량40 reactions

역전사 효소ArrayScript™ UP

배송 조건Wet Ice

충분40 Reactions

용도(애플리케이션)Gene Expression

반응 수40 Reactions

샘플 종류RNA

Unit SizeEach