Thermo Fisher Scientific Human Sonic Hedgehog C25II Recombinant Protein, PeproTech
Recombinant human Sonic Hedgehog C25II protein expressed in E. coli. High purity (≥98%) and low endotoxin (<1 EU/µg). Functional in inducing alkaline phosphatase in C3H/10T1/2 cells. Lyophilized form for research use only.
- 카탈로그번호
- 100-45-xxxxx (5개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
5개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Human Sonic Hedgehog C25II Recombinant Protein, PeproTech
Applications
Western Blot (WB)
- Tested Dilution: Not specified
- Publications: View 1 publication
Functional Assay
- Assay-dependent
- Publications: None listed
In Vitro Assay (IV)
- Tested Dilution: Not specified
- Publications: View 32 publications
Miscellaneous (PubMed)
- Publications: View 3 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published species | Human, Mouse, Xenopus |
| Expression System | E. coli |
| Amino Acid Sequence | IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
| Molecular Weight | 20 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 98% (SDS-PAGE & HPLC) |
| Endotoxin Concentration | <1 EU/µg |
| Activity | Induces alkaline phosphatase in C3H/10T1/2 (CCL-226) cells. Expected ED50: 0.8–1.0 µg/ml |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
- Catalog No. 100-45-1MG is provided as 2 × 500 µg (100-45-500UG).
- Recombinant E. coli-derived human Sonic Hedgehog is a 20 kDa protein with 176 amino acids, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring, chemically modified Cys residue.
- Shipped at ambient temperature. For storage, handling, and reconstitution details, refer to the lot-specific Certificate of Analysis.
Target Information
Sonic Hedgehog (SHH) is expressed during embryogenesis and plays a critical role in early developmental patterning, including ventral neural tube, limb axis, and somite formation.
The human SHH protein is the most similar to the Drosophila counterpart and is produced as a precursor that undergoes autocatalytic cleavage. The N-terminal fragment provides signaling activity, while the C-terminal fragment assists in processing and covalently attaches a cholesterol moiety to the N-terminal product, restricting diffusion within the embryo.
Defects in SHH or its pathway can cause holoprosencephaly (HPE), leading to forebrain and facial malformations, and may also contribute to VACTERL syndrome, characterized by multiple congenital abnormalities.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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