Thermo Fisher Scientific GbetaL Polyclonal Antibody
Western blot 및 Immunoprecipitation에 사용 가능한 Rabbit Polyclonal 항체. Human 및 Mouse에 반응하며, mTOR 신호전달 관련 GbetaL 단백질을 타겟으로 함. 항원 친화성 크로마토그래피로 정제된 액상 형태의 1 mg/mL 항체로, 4°C에서 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 275 and the C-terminus (residue 326) of human G protein Beta subunit-like |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Rat, Zebrafish, X. laevis, X. tropicalis, and Bovine.
Target Information
G-protein beta subunit like (GBL protein) is a novel member of the WD-40 protein family. It is a highly conserved cytosolic protein consisting of seven WD-40 repeating motifs.
LST8 is a functional component of the mTOR signaling complex (TORC1) and positively regulates the mTOR pathway. It interacts with the kinase domain of mTOR and stabilizes its interaction with raptor.
LST8 participates in regulating cell growth and in nutrient and growth factor-mediated mTOR signaling to S6K1. Reports suggest that LST8 expression is up-regulated by insulin, indicating a possible role in intracellular events related to insulin-receptor activation, including insulin-stimulated glucose transport and glycogen synthesis.
Northern blot analysis detected ubiquitous expression of LST8, with highest levels in brain and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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