Thermo Fisher Scientific Phospho-Cyclin B1 (Ser126) Polyclonal Antibody
Cyclin B1의 Ser126 인산화 형태를 특이적으로 검출하는 polyclonal antibody. Western blot, IHC, ICC/IF에 사용 가능. Rabbit IgG 기반, 1 mg/mL 농도, -20°C 보관. 세포 주기 조절 및 암 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human CCNB1 (Accession P14635), around phosphorylated Ser126 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816268 |
Product Specific Information
Antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Serine 126.
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein essential for control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF).
Two alternative transcripts exist: a constitutively expressed form and a G2/M phase-regulated form. Cyclin B1 accumulates during G2 phase and is degraded at the end of mitosis via APC-dependent destruction, which is required for cell cycle progression.
Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: PA5-104795_Cyclin_B1_P14635-1_Rabbit.svg, PA5-104795_Cyclin_B1_P14635-1_Rabbit_PDP.jpeg)
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