Thermo Fisher Scientific GLI2 Monoclonal Antibody (OTI1F9), TrueMAB
GLI2 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot 및 IHC(Paraffin) 적용 가능. 인간 GLI2(aa 729–1123) 재조합 단백질로 면역생성. 동결건조 형태, PBS/trehalose buffer에 보관. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 729–1123 of human GLI2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass act as transcription factors binding DNA via zinc finger motifs with conserved H-C links.
Gli family proteins mediate Sonic hedgehog (Shh) signaling and function as oncogenes in embryonal carcinoma cells.
The protein localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression, playing a role in embryogenesis.
Associated phenotypes include Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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