Thermo Fisher Scientific FBXO33 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human FBXO33. Recombinant protein control fragment (Product #RP-102199). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FBXO33,uniProtId:Q7Z6M2-1,ncbiNodeId:9606,antigenRange:198-326,antigenLength:555,antigenImageFileName:PA5-61275_FBXO33_Q7Z6M2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-61275_FBXO33_Q7Z6M2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2641546

Product Specific Information

Immunogen sequence: NNRNLQKFSL FGDISVLQQQ GSLSNTYLSK VDPDGKKIKQ IQQLFEEILS NSRQLKWLSC GFMLEIVTPT SLSSLSNAVA NTMEHLSLLD NNIPGNSTLI TAVELERFVN LHSLALDFCD FTAEMARVL

Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 95%.

Target Information

FBXO33 is the substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. FBXO33 probably recognizes and binds to phosphorylated target proteins. Recognizes YBX1.Members of the F-box protein family, such as FBXO33, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.