Thermo Fisher Scientific CNOT4 Monoclonal Antibody (OTI1H2), TrueMAB
CNOT4 단백질을 인식하는 Mouse monoclonal antibody로, Western blot 및 IHC(P)에서 검증됨. Human 시료에 반응하며, E3 ubiquitin ligase 관련 연구에 적합. 고순도 Affinity chromatography 정제, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 190–455 of human CNOT4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CNOT4 is a 575 amino acid protein with E3 ubiquitin ligase activity, mainly involved in protein modification and ubiquitination. It contains a C4C4-type zinc finger domain, an RNA recognition motif, and a bipartite nuclear localization signal.
CNOT4 is a component of the CCR4-NOT (CAF1) core complex that functions in general transcription regulation, exerting both positive and negative effects on transcription of multiple genes, thereby controlling gene expression.
CNOT4 interacts with CNOT1 via its C-terminus and with E2 ubiquitin ligases via the C4C4 RING domain. It is reported to be phosphorylated in response to DNA damage at consensus sites recognized by ATM and ATR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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