Thermo Fisher Scientific EN2 Polyclonal Antibody
EN2 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 다클론 항체. Western blot 및 Immunocytochemistry에 적합하며, 인간과 생쥐에 반응. 합성 펩타이드 면역원 기반으로 제조되어 높은 특이성과 재현성을 제공.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (NHSTTAKEGKSDSE) corresponding to the C-terminus amino acids of EN2 (aa 320–333) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_10980491 |
Product Specific Information
- This antibody is predicted to react with canine, human, and mouse based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 4,000.
Target Information
Homeobox-containing genes are thought to have a role in controlling development.
In Drosophila, the engrailed (en) gene plays an important role during segmentation, required for formation of posterior compartments.
Different mutations in the mouse homologs, En1 and En2, produce developmental defects that can be lethal.
The human engrailed homologs 1 and 2 encode homeodomain-containing proteins implicated in pattern formation during development of the central nervous system.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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