Thermo Fisher Scientific Nucleophosmin Recombinant Mouse Monoclonal Antibody (rNPM1, 1901)
Nucleophosmin 1 (NPM1) 단백질을 표적하는 Thermo Fisher Scientific의 재조합 마우스 단일클론 항체. Western blot, IHC, Flow cytometry에 적합하며, 높은 특이성과 재현성을 제공. HEK293 발현 시스템 기반, Protein A/G 정제, 연구용 전용 제품.
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Thermo Fisher Scientific Nucleophosmin Recombinant Mouse Monoclonal Antibody (rNPM1, 1901)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rNPM1, 1901 |
| Immunogen | Recombinant human NPM1 protein fragment (aa185–287) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
NPM1 (Nucleophosmin 1, also known as B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly and transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and p53 regulation.
It shuttles between the nucleus, cytoplasm, and nucleolus, chaperoning core histones and preventing protein aggregation.
NPM1 interacts with tumor-associated chromosome translocations, forming fusion proteins such as NPM-ALK, NPM-RAR, and NPM-MLF1, which may contribute to oncogenic processes.
Dysfunction of NPM1 is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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