Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, AP

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:50,000

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

AP AP AP

View additional formats

• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Donkey/)
• [DyLight 350](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 350/host/Donkey/)
• [DyLight 488](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 488/host/Donkey/)
• [DyLight 550](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Donkey/)
• [DyLight 594](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 594/host/Donkey/)
• [DyLight 650](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Donkey/)
• [DyLight 680](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 680/host/Donkey/)
• [DyLight 755](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 755/host/Donkey/)
• [DyLight 800](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Donkey/)
• [FITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/FITC/host/Donkey/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Donkey/)
• [Rhodamine](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Rhodamine/host/Donkey/)
• [TRITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Donkey/)
• Request custom conjugation

Form

Lyophilized

Concentration

0.6 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 8, with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_228211

Target

IgG

Cross Adsorption

Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep serum proteins

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

This antibody has been successfully used in Western blot and IP applications.

Antibody Specificity: This antibody reacts with the heavy chains on rabbit IgG and with the light chains common to most rabbit immunoglobulins, based on electrophoresis. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Restoration and Storage: Store product at 2-8°C until opened. Restore with 0.5 mL distilled water (0.6 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 2-8°C as an undiluted liquid. After dilution, do not use for more than one day.

To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.

Country of Origin: USA

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.