Thermo Fisher Scientific RanGAP1 Polyclonal Antibody
RanGAP1 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal antibody. Western blot, IHC, IP 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 액상 형태이며, 4°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 100 and 150 of Human Ran GTPase activating protein 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Target Information
Ran is a small signaling GTPase involved in nucleocytoplasmic transport. It also plays roles in spindle aster formation and nuclear envelope reassembly during mitosis. Unlike Ras or Rho, Ran is not membrane-associated. The localization of RanGAP and RCC1 defines the RanGTP/RanGDP gradient essential for signaling. Mammalian RanGAP binds to the nuclear pore via SUMOylation and interaction with nucleoporin Nup358.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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