Thermo Fisher Scientific c-Mpl Monoclonal Antibody (1H2)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA5-17127 targets MPL in indirect ELISA, FACS, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human MPL expressed in E. coli.
MA5-17127 detects MPL with a predicted molecular weight of approximately 71.2 kDa.

Target Information

In 1990, an oncogene v-mpl was identified from the murine myeloproliferative leukemia virus, capable of immortalizing bone marrow hematopoietic cells from different lineages.
In 1992, the human homologue c-mpl was cloned, encoding a protein homologous to members of the hematopoietic receptor superfamily.
Anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation.
The ligand for c-mpl, thrombopoietin, cloned in 1994, was shown to be the major regulator of megakaryocytopoiesis and platelet formation.
The protein encoded by c-mpl, CD110, is a 635 amino acid transmembrane protein with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs.
TPO-R deficient mice are severely thrombocytopenic, emphasizing the crucial role of CD110 and thrombopoietin in megakaryocyte and platelet formation.
Upon thrombopoietin binding, CD110 dimerizes, leading to phosphorylation of JAK and STAT family kinases, MAPK, adaptor protein Shc, and the receptors themselves.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.