Thermo Fisher Scientific Phospho-ATG4D (Ser467) Polyclonal Antibody
Phospho-ATG4D (Ser467)에 특이적인 Rabbit Polyclonal Antibody로, 인간·생쥐·랫드 시료에 반응합니다. WB, IHC, ICC/IF, ELISA 등 다양한 응용 가능. Protein A로 정제된 액상 항체이며 -20°C에서 보관. 연구용으로만 사용.
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Applications 및 권장 희석 배수
| 응용 분야 | 권장 희석 배수 |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human ATG4D around the phosphorylation site of Ser467 [RP(p-S)SE] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Atg4D (autophagy-related gene 4D), also known as APG4D or AUTL4, is a 474 amino acid protein localized in the cytoplasm and belongs to the C-54 family of cysteine proteases. It is predominantly expressed in skeletal muscle and testis.
Atg4D functions as a cysteine protease required for autophagy, cleaving the C-terminal region of target proteins to enable their binding to autophagosomes. Its enzymatic activity is inhibited by N-ethylmaleimide, a thiol-reactive compound that modifies cystine residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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