Thermo Fisher Scientific beta-Actin Polyclonal Antibody
다양한 종에 반응하는 Rabbit Polyclonal β-Actin 항체로 Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 세포 골격 연구 및 단백질 발현 정량의 로딩 컨트롤로 적합. 고순도 Protein A 정제 및 안정한 보존용액 구성.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | All, Bovine, Dog, Goat, Human, Mouse, Sheep, Pig, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MAP conjugated synthetic peptide derived from human beta Actin, amino acids 1–200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein expressed in all human cell types and plays a crucial role in cell motility, structure, and integrity. Beta and gamma actins coexist as cytoskeletal components in most cell types. Polymerization of globular actin (G-actin) forms filamentous actin (F-actin) in a two-stranded helical structure. Due to its ubiquitous expression, beta actin is commonly used as a loading control in protein detection assays such as Western blotting. Antibodies to beta-actin are valuable tools for studying intracellular distribution and cytoskeletal dynamics.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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