Thermo Fisher Scientific Aconitase 1 Recombinant Rabbit Monoclonal Antibody (22C10)
Aconitase 1 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot, ICC/IF, Flow cytometry, ELISA에 활용 가능. 인간 시료에 반응하며, HEK293 세포에서 발현된 IgG 형태. 철 항상성 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 22C10 |
| Immunogen | A synthesized peptide derived from human ACO1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3092519 |
Target Information
Aconitase 1 (ACO1), also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein that binds to iron-responsive elements (IREs). It plays a central role in cellular iron homeostasis.
IREs are stem-loop structures found in the 5′ UTR of ferritin mRNA and in the 3′ UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of ferritin mRNA translation and inhibition of transferrin receptor mRNA degradation.
Thus, IREB1 plays a key role in iron metabolism and also exhibits aconitase enzymatic activity, grouping it within the aconitase enzyme family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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