Thermo Fisher Scientific ETV4 Monoclonal Antibody (OTI1E11), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882) to ensure carrier-free conditions.

Target Information

ETV4 (E1A enhancer binding protein, E1AF), also known as PEA3, is a member of the Ets gene family encoding sequence-specific DNA-binding proteins.
Ets family members (e.g., PU.1, Ets-1, Ets-2, GABP-α) recognize DNA motifs containing a central 5'-GGAA-3' element.
PEA3 specifically binds the motif 5'-AGGAAG-3' but not 5'-AGGAAC-3', showing distinct sequence specificity among Ets proteins.
PEA3 is expressed in epithelial and fibroblastic cells but not in hematopoietic cells, contrasting with other Ets family proteins such as Ets-1, Ets-2, and Fli-1, which are primarily expressed in hematopoietic lineages.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.