Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience

Applications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available upon request

Product Specific Information

• Description: Monoclonal rat IgG2a, kappa isotype control immunoglobulin, useful as an isotype control antibody.
• Applications Reported: Suitable for flow cytometric analysis and ELISA.
• Applications Tested: Used for flow cytometric analysis of mouse splenocyte suspension; recommended at same concentration as experimental antibody.
• Storage and Handling: Use in a sterile environment.
• Filtration: 0.2 µm post-manufacturing filtered.
• Purity: >90% (SDS-PAGE).
• Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
• Aggregation: <10% (HPLC).

Target Information

Rat IgG2a is an immunoglobulin subclass produced by the rat immune system. It activates complement and mediates antibody-dependent cell-mediated cytotoxicity (ADCC).

• Molecular weight: ~160 kDa
• Structure: Two heavy chains and two light chains
• Common uses: Control or reference antibody in rat model experiments, flow cytometry, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.