Thermo Fisher Scientific SPHK2 Monoclonal Antibody (3C8D3)
인간 SPHK2 단백질을 인식하는 Mouse IgG1 단클론 항체로, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. Protein G 정제, PBS 버퍼 및 sodium azide 함유. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3C8D3 |
| Immunogen | Synthesized peptide of human SK2 (AA: 36–52) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787528 |
Product Specific Information
MA5-31905 has been tested in indirect ELISA.
Target Information
SPHK2, along with SPHK1, catalyzes the phosphorylation of sphingosine producing sphingosine-1-phosphate (S1P), a signaling molecule that regulates diverse cellular processes through G-protein coupled receptors. Inhibitors of SPHKs are considered potential therapeutics for cancer treatment. SPHK2 plays roles in cell differentiation, calcium mobilization, and apoptosis. S1P receptors (EDG family) mediate various cellular responses such as motility, survival, migration, and cell-cell interaction. S1P also contributes to cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, and neuronal and bone cell regulation. S1P levels are controlled by Sphk1 and Sphk2 activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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