Thermo Fisher Scientific HMGN3 Polyclonal Antibody
HMGN3 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, IP 등에 적합하며 인간 시료에 반응. 항원 친화 크로마토그래피로 정제된 액상 형태로, 4°C에서 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 60 and 99 of human high mobility group nucleosomal binding domain 3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
HMGN3 binds to nucleosomes, regulating chromatin structure and chromatin-dependent processes such as transcription, DNA replication, and DNA repair. It affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion. HMGN3 regulates the expression of the glucose transporter SLC2A2 by binding to its promoter region and recruiting PDX1 and other transcription factors. It also regulates SLC6A9, a glycine transporter involved in synaptic glycine concentration in the CNS, by binding to its transcription start site. May play a role in ocular development and astrocyte function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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