Thermo Fisher Scientific BRAF Monoclonal Antibody (OTI1G8), TrueMAB
BRAF 단백질을 인식하는 마우스 모노클로날 항체로, Western blot 및 IHC(P) 실험에 적합합니다. Human 시료 반응성이 있으며, 동결건조 형태로 제공됩니다. MAPK 신호전달 연구 및 암 관련 단백질 분석에 활용 가능합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G8 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human BRAF expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer and is thought to play a role in postsynaptic responses of hippocampal neurons.
This protein regulates the MAP kinase/ERKs signaling pathway, influencing cell division, differentiation, and secretion.
Mutations in BRAF are associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and lung adenocarcinoma.
A pseudogene for BRAF is located on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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