Thermo Fisher Scientific pan T cells Monoclonal Antibody (OX-52), Biotin

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Flow Cytometry (Flow)

0.5 µg/1x10^6 cells

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OX-52

Immunogen

Immunogen:PVG-RT1c thoracic duct lymphocytes and spleen cells

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• FITC
• Request custom conjugation

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 4-5mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538921

Product Specific Information

Anti-rat pan T lymphocyte monoclonal antibody immunoprecipitates a two chain structure (95, 120 kDa) largely restricted to T lymphocytes and thymocytes. Applications therefore include the identification of T lymphocyte lineage cells in suspension and tissue. The antibody has previously been used to stain T cell areas of the spleen, lymph nodes and Peyer`s patch. In the thymus, this antibody labels all thymocytes, however medullary cells are more strongly positive than cortical cells. The antibody does not label such non-lymphoid tissues such as brain, kidney, liver and skin. This antibody stains approximately 1.0% of bone marrow cells, and 56% thoracic duct lymphocytes. Weak staining occurs with 50% dendritic cells from thoracic duct of mesenteric lymphadenectomized rats. The antigen recognized by this antibody is not expressed on granulocytes or macrophages. The function of the antigen recognized by this antibody has not, as yet, been associated with any particular function of T cells. The antibody does not inhibit the allogeneic mixed leukocyte response, nor does it inhibit T cytotoxic effector cell function. This clone works in flow cytometry, Immunoprecipitation and with frozen sections.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.