Thermo Fisher Scientific P4HA2 Monoclonal Antibody (CL0351)
인간 P4HA2 단백질을 인식하는 CL0351 모노클로날 항체로, WB 및 IHC(P) 실험에 적합합니다. 단백질 A로 정제된 비결합형 액상 항체이며, 고순도와 높은 특이성을 제공합니다. 콜라겐 합성 관련 연구용으로 최적화되어 있습니다.
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Thermo Fisher Scientific P4HA2 Monoclonal Antibody (CL0351)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CL0351 |
| Immunogen | Recombinant protein corresponding to Human P4HA2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2637214 |
Product Specific Information
Immunogen sequence:
LKEYILVEEA KLSKIKSWAN KMEALTSKSA ADAEGYLAHP VNAYKLVKRL NTDWPALEDL VLQDSAAGFI ANLSVQRQFF PTDEDEIGAA KALMRLQDTY RLDPGTISRG ELPGTKYQAM LSVDDCFGM
Highest antigen sequence identity to orthologs:
- Mouse: 90%
- Rat: 91%
Binds to an epitope located within the peptide sequence NTDWPALEDLVLQDS, as determined by overlapping synthetic peptides.
Target Information
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein provides the major part of the catalytic site of the active enzyme.
In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline, essential for proper three-dimensional folding of newly synthesized procollagen chains.
Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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