Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), FITC, eBioscience
FITC 결합 IL-17A 단클론 항체로 마우스 및 랫트 IL-17A를 특이적으로 인식하며 IL-17F는 인식하지 않음. Flow cytometry 등 다양한 응용 가능. 염증성 사이토카인 연구 및 Th17 세포 분석에 적합. 4°C 암암 저장, 동결 금지.
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Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 120 publications |
| ELISA | - | View 3 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio17B7 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1834374 |
Available Formats
- Alexa Fluor 488
- APC
- Biotin
- Brilliant UV 395 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 650 / 711 / 786
- eFluor 450 / 506 / 660
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Custom conjugation available
Product Specific Information
The eBio17B7 antibody reacts with mouse and rat IL-17A without recognition of IL-17F.
IL-17A is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory conditions such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
Human IL-17A exists as glycosylated 20–30 kDa homodimers produced by activated CD4+ T cells. It enhances ICAM-1 expression in fibroblasts and stimulates epithelial, endothelial, and fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of IL-17A, fibroblasts can sustain proliferation of CD34+ progenitors and induce neutrophil maturation. Mouse, rat, and human IL-17A induce IL-6 secretion in mouse stromal cells, indicating cross-species receptor recognition.
IL-23-dependent IL-17A-producing CD4+ T cells (Th17 cells) represent a distinct subset regulated negatively by IFN-γ and IL-4.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested by intracellular staining and flow cytometry of restimulated, Th17-polarized splenocytes using the Fixation and Permeabilization Buffer Set (Product #88-8824-00). Recommended ≤0.25 µg/test (100 µL final volume; 10⁵–10⁸ cells/test).
Additional Protocols: Compatible with Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Excitation: 488 nm; Emission: 520 nm; Laser: Blue.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a 32 kDa disulfide-linked homodimer (136 amino acids) that signals through IL-17R/CDw217. It regulates NF-κB and MAPK pathways, stimulates IL-6 and COX-2 expression, and enhances nitric oxide production. IL-17A is crucial in inflammatory and autoimmune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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