Thermo Fisher Scientific IL-12 p70 Monoclonal Antibody (9A5)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 7 publications 7 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Neutralization (Neu)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Pig, Rat

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

9A5

Immunogen

Recombinant mouse IL-12

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Caprylic acid precipitation

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223525

Product Specific Information

MM120 targets IL-12 in ELISA, and WB applications and shows reactivity with mouse samples.

The MM120 immunogen is recombinant mouse IL-12.

MM120 detects IL-12 which has a predicted molecular weight of approximately 22 kDa.

The MM120 mouse IL12 antibody (clone 9A5) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM121B (biotinylated conjugate of clone C17-8). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody MM120 (clone 9A5) and biotinylated antibody MM121B (clone C17-8) have successfully been used in combination with recombinant mouse IL12p70 protein SMIL12 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system`s response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.