Thermo Fisher Scientific Peroxidase Polyclonal Antibody
Western blot, IHC, ELISA, IP 등 다양한 응용에 적합한 과산화효소 폴리클로날 항체. 기니피그 유래 항체로 HRP 검출에 높은 특이성과 안정성을 제공. 동결 건조 형태로 장기 보관 가능하며 연구용으로 사용.
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- 100-2138
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Guinea pig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Peroxidase antibody produced by repeated immunization of guinea pigs with peroxidase isolated from horseradish |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 85 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid repeated freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-guinea pig serum, purified and partially purified Peroxidase (Horseradish).
Cross-reactivity against peroxidase from other tissues and species may occur but has not been specifically determined.
Target Information
Horseradish peroxidase (HRP) is an enzyme commonly used in biochemistry and molecular biology research.
It catalyzes the oxidation of various substrates in the presence of hydrogen peroxide.
HRP is widely used as a detection enzyme in immunoassays such as ELISA and Western blot due to its high sensitivity and stability.
It can also serve as a tracer in enzyme-linked immunosorbent assays and as a labeling agent in fluorescent microscopy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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