Invitrogen™

RNAqueous™-4PCR Total RNA Isolation Kit

The RNAqueous™-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 mg–75 mg) or from cells자세히 알아보기

The RNAqueous™-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 mg–75 mg) or from cells (∼100 cells to ∼1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-_free_™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. Features of this kit:

• DNA-free RNA for RT-PCR
• Rapid, phenol-free total RNA isolation
• Novel reagent for rapid and safe removal of DNase and divalent cations

Using the RNAqueous™-4PCR Kit
The RNAqueous™-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNA_later_™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. There is no need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.

사양

Isolation TechnologySilica Spin Column

정제 시간10 min. (After sample homogenization)

용리량50–120 μL

샘플 종류Tissue, Cells

최종 제품 유형Total RNA

고처리량 호환성Not High-throughput Compatible (Manual)

용도(애플리케이션)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling

수량30 Preps

배송 조건Box 1: Dry Ice
Box 2: Room Temperature

시작 물질 양Tissue: ≤75 mg
Cells: ≤107

수율≤10 μg per 1 mg tissue
≤1 μg per 1 x 105 cells

Unit SizeEach