Thermo Fisher Scientific RNAqueous-4PCR Total RNA Isolation Kit
페놀을 사용하지 않고 조직 및 세포에서 총 RNA를 신속하게 분리하는 키트. DNA-free 시약 포함으로 RT-PCR에 적합. RNA_later™와 함께 사용 시 조직 보존 및 간편한 처리 가능. 최대 10 µg RNA 수율로 다양한 분자생물학 응용에 활용.
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Invitrogen™ RNAqueous™-4PCR Total RNA Isolation Kit
The RNAqueous™-4PCR Kit enables phenol-free total RNA isolation from small tissue samples (0.5 mg–75 mg) or from cells (~100 cells to ~1 × 10⁷ cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
DNA-_free_™ DNA removal reagents are included, making the isolated RNA especially suitable for RT-PCR.
주요 특징
- DNA-free RNA for RT-PCR
- Rapid, phenol-free total RNA isolation
- Novel reagent for rapid and safe removal of DNase and divalent cations
사용 안내
The RNAqueous™-4PCR Kit is designed for isolation of RNA for applications such as RT-PCR, benefiting from its strong genomic DNA removal capability.
When used with RNA_later_™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and easily disrupted in standard microfuge tubes using the pestles provided in the kit.
There is no need for snap-freezing in liquid nitrogen or grinding with a mortar and pestle.
사양 (Specifications)
| 항목 | 내용 |
|---|---|
| Isolation Technology | Silica Spin Column |
| 정제 시간 | 10 min (After sample homogenization) |
| 용리량 | 50–120 μL |
| 샘플 종류 | Tissue, Cells |
| 최종 제품 유형 | Total RNA |
| 고처리량 호환성 | Not High-throughput Compatible (Manual) |
| 용도 (Applications) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
| 수량 | 30 Preps |
| 배송 조건 | Box 1: Dry Ice / Box 2: Room Temperature |
| 시작 물질 양 | Tissue: ≤75 mg / Cells: ≤10⁷ |
| 수율 | ≤10 μg per 1 mg tissue / ≤1 μg per 1 × 10⁵ cells |
| Unit Size | Each |
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