Thermo Fisher Scientific RNAqueous-96 Total RNA Isolation Kit
고처리량 샘플에서 페놀 없이 RNA를 정제하는 키트로, 세포 및 조직 시료에 적합합니다. 유리섬유 필터 기술로 높은 수율과 순도를 제공합니다. RT-PCR, qPCR 등 다양한 분자생물학 응용에 사용 가능합니다. DNase 처리 옵션 포함.
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Invitrogen™ RNAqueous™-96 Total RNA Isolation Kit
The RNAqueous™-96 Kit is designed for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
The kit contains sufficient reagents for 192 (2 × 96) RNA purifications and is ideal for isolating total RNA from multiple or very small samples.
Using the RNAqueous™-96 Kit
The procedure employs an RNA-binding glass-fiber filter and uses either vacuum filtration or centrifugation methods to achieve high yields of intact RNA.
An optional on-the-filter DNase treatment can be performed to remove genomic DNA, ensuring RNA purity for RT-PCR and other downstream applications.
The 96-well plates are compatible with most common 96-well plate vacuum manifolds and centrifuge rotors, providing consistent, high-quality RNA yields from diverse starting materials.
Accessory Product
- RNAqueous™-96 Automated Kit (Cat. No. AM1812): Same chemistry, optimized for automated platforms.
제품 사양
| 항목 | 내용 |
|---|---|
| Isolation Technology | Silica Plate |
| Elution Volume | ≥50 µL |
| Sample Type | Cells, Tissue |
| Final Product Type | Total RNA |
| Throughput Compatibility | High-throughput Compatible |
| Instrument Compatibility | MultiPROBE™ II HT |
| Applications | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
| Quantity | 192 Preps |
| Shipping Conditions | Box 1: Dry Ice / Box 2: Room Temperature |
| Starting Material | Cells: ≤1×10⁶, Tissue: ≤1.5 mg |
| Unit Size | Each |
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