Thermo Fisher Scientific TNNT2 Monoclonal Antibody (OTI7B1), TrueMAB
Cardiac Troponin T 단백질을 인식하는 Monoclonal Antibody로, Western Blot과 IHC(P)에서 검증됨. Human 시료에 반응하며, Mouse IgG2a 아이소타입. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도 유지. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7B1 |
| Immunogen | Full length human recombinant protein of human TNNT2 produced in E. coli (Target Family: Cardiac Troponin T, UniProt ID: P45379-1, Antigen Range: 1–298, Antigen Length: 298) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Cardiac Troponin T is the tropomyosin-binding subunit of the troponin complex located on the thin filament of striated muscles. It regulates muscle contraction in response to intracellular calcium ion concentration changes and is essential for muscle function and myofibril formation.
In the human heart, four cTnT isoforms are developmentally regulated. Expression levels are influenced by heart failure. Mutations in Cardiac Troponin T are linked to familial hypertrophic and dilated cardiomyopathies. The gene undergoes alternative splicing, producing tissue-specific isoforms.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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