Thermo Fisher Scientific CD55 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 2.5 µg per million cells

Product Specifications

Product Specific Information

In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human CD55 and recombinant mouse CD97 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from the classical and alternative pathways of the complement cascade.
Bidirectional interactions between CD55 and CD97 are involved in T cell regulation, and CD55 can regulate complement when bound to CD97.
In tumors, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness, and evasion of apoptosis.

CD55 is a ~70 kDa glycoprotein (in erythrocytes) anchored in the membrane by a glycosylphosphatidylinositol tail. In other cells, the apparent molecular weight is slightly larger and has a substantial content of O-glycans.
CD55 binds to activated C4b or C3b complement fragments on the cell surface, preventing assembly and accelerating decay of both classical and alternative pathways.
CD55 carries the Cromer-related blood group antigens.

Two alternatively spliced transcripts encoding CD55 have been identified:

1. A predominant transcript encoding a membrane-bound protein expressed on cells exposed to plasma component proteins.
2. An alternatively spliced transcript producing a soluble protein present at much lower levels.

CD55 has been studied for its role in prostate cancer growth and survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.