Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Recombinant Rabbit Monoclonal Antibody (HisH3S10-4B6), APC
Phospho-Histone H3 (Ser10)을 인식하는 재조합 토끼 단클론 항체로, APC 형광 표지되어 있습니다. Flow Cytometry에 최적화되어 있으며, 높은 특이성과 재현성을 제공합니다. PBS 버퍼에 보관되며 4°C에서 안정적입니다.
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Applications
- Flow Cytometry (Flow): 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HisH3S10-4B6 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser10 of human phospho histone H3 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896887 |
Additional Formats
- Unconjugated (MA5-36949)
- FITC (MA5-36951)
- PE (MA5-36950)
- Custom conjugation available upon request
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histone proteins, it forms the nucleosome structure that organizes DNA. Histones play key roles in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of histone H3, such as methylation, acetylation, and phosphorylation (including at Ser10), influence chromatin structure and gene expression by altering transcription factor binding and chromatin accessibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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