Thermo Fisher Scientific Phospho-IRAK1 (Thr100) Polyclonal Antibody
인간 IRAK1 Thr100 인산화 부위에 특이적인 폴리클로날 항체로, Western blot 및 IHC(Paraffin) 분석에 적합. Rabbit IgG 호스트, 비결합형 액상 형태로 제공. 세포 내 신호전달 연구 및 TLR/IL-1 경로 분석에 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
- View 1 publication
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:100
- View publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr100 (I-I-TP-A-W) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2555226 |
Target Information
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK’s association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activation of type I IFN genes, which drive the cell into an antiviral state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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