Thermo Fisher Scientific RET Monoclonal Antibody (OTI1G1), TrueMAB
RET 단백질을 인식하는 Mouse 모노클로날 항체로 Western Blot, IHC, ICC 등 다양한 분석에 적합함. 인간 시료 반응성, 고순도 친화 크로마토그래피 정제, 무보존제 PBS 버퍼 제공. 신경능선 발달 및 종양 관련 RET 연구용으로 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 713–1017 of human RET produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
RET (rearranged during transfection) is a receptor tyrosine kinase in the cadherin superfamily. It plays a vital role in neural crest development and can be activated oncogenically by cytogenetic rearrangements. RET mediates cell growth and differentiation signals.
Mutations in the RET gene are linked to multiple endocrine neoplasia type IIA and IIB, Hirschsprung disease, and medullary thyroid carcinoma.
Two transcript variants encoding distinct isoforms have been identified, with additional variants of uncertain biological validity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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