Thermo Fisher Scientific UBE2T Monoclonal Antibody (OTI2F5)
UBE2T 단백질을 인식하는 Mouse Monoclonal Antibody로 Western blot, IHC, ICC/IF에 사용 가능. Human 시료에 반응하며, Affinity Chromatography로 정제됨. PBS 기반 용액 형태로 제공되며, -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100 | View publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F5 |
| Immunogen | Full length human recombinant protein of UBE2T produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.33 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2725616 |
Target Information
UBE2T is involved in the covalent conjugation of ubiquitin to proteins, regulating diverse cellular pathways and protein functions. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2, such as UBE2T), and a ubiquitin ligase (E3).
UBE2T catalyzes the covalent attachment of ubiquitin to protein substrates, accepts ubiquitin from the E1 complex, and catalyzes its covalent attachment to other proteins. It is involved in mitomycin-C (MMC)-induced DNA repair and catalyzes monoubiquitination of FANCD2, a key step in the DNA damage pathway. Defects in the UBE2T gene are associated with Fanconi anemia of complementation group T.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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