Thermo Fisher Scientific hnRNP M3/4 Polyclonal Antibody
Thermo Fisher Scientific의 hnRNP M3/4 Polyclonal Antibody는 인간 및 생쥐 반응성을 가진 Rabbit IgG 폴리클로날 항체로, Western blot, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 1 mg/mL 농도를 유지합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 475 and 525 of human Heterogeneous Nuclear Ribonucleoprotein M (UniProt ID: P52272-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
Proteins that directly bind to nascent RNA polymerase II transcripts, the heterogeneous nuclear ribonucleoproteins (hnRNPs), play an important role in transcript-specific packaging and alternative splicing of pre-mRNAs.
The M1–M4 hnRNP proteins form a cluster and act as pre-mRNA binding proteins in vivo, binding strongly to poly(G) and poly(U) RNA homopolymers in vitro.
These proteins belong to the ribonucleoprotein consensus sequence family and contain a methionine-arginine–rich hexapeptide repeat motif (MR repeat), similar to the 64 kDa subunit of cleavage stimulation factor involved in 3'-end mRNA processing.
Immunologically related M proteins are found across eukaryotes, from humans to yeast.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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