Thermo Fisher Scientific IDO Monoclonal Antibody (C14)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.01-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5-30 µg/mL

-

Immunocytochemistry (ICC/IF)

5-30 µg/mL

-

Product Specifications

Species Reactivity

Human, Pig

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

C14

Immunogen

Recombinant Human IDO protein, Ala2-Gly403 (Accession #P14902) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IDO,uniProtId:P14902-1,ncbiNodeId:9606,antigenRange:2-403,antigenLength:403,antigenImageFileName:MA5-50873_IDO_P14902-1_House_mouse.svg,antigenImageFileNamePDP:MA5-50873_IDO_P14902-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.05% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3090577

Target Information

IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin. By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction. The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.