Thermo Fisher Scientific H4K8ac Polyclonal Antibody
H4K8ac 폴리클로날 항체로서 인간, 마우스, 랫트 시료에 반응합니다. Western blot, IHC, ICC/IF, ELISA, ChIP 등 다양한 응용에 적합합니다. 합성 아세틸화 펩타이드 항원으로 제작되었으며, 액상 형태로 -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
| ChIP assay (ChIP) | 5 µg antibody for 5–10 µg of chromatin |
| ChIP-sequencing (ChIP-Seq) | 1:20–1:50 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic acetylated peptide around K8 of human Histone H4 (NP_0035291) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.49 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901723 |
Product Specific Information
- Positive samples: 293T, NIH/3T3, C6
- Immunogen sequence: GGKGL
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes. Two molecules each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in chromatin compaction into higher-order structures. This gene, part of the histone H4 family, is intronless and located within the large histone gene cluster on chromosome 6. Transcripts lack polyA tails and contain a palindromic termination element.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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