Thermo Fisher Scientific CD357 (AITR/GITR) Chimeric Recombinant Rat Monoclonal Antibody (DTA-1)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Specificity:
This antibody is specific for murine GITR, a member of the tumor necrosis factor receptor (TNFR) family that is expressed at low levels on unstimulated T cells, B cells, and macrophages. Upon activation, CD4+ and CD8+ T cells up-regulate GITR expression, whereas immunoregulatory T cells constitutively express high levels of GITR.

Preparation:
This antibody was raised by immunising Wistar rats with the CD25+CD4+ T cell line. Hybridoma cells were generated by fusing spleen cells from the immunized rat with P3X63Ag8.653 myeloma cells, assessed for secreting antibodies capable of neutralizing in vitro CD25+CD4+ T cell-mediated suppression of anti-CD3-stimulated T cell proliferation, and then cloned. The DTA-1 clone secreted a monoclonal rat immunoglobulin G2a (IgG2a) with high neutralizing activity.

Target Information

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also known as GITR or AITR, is a protein encoded by the TNFRSF18 gene in humans (mapped to 1p36.33). This gene encodes a member of the TNF-receptor superfamily. The receptor shows increased expression upon T-cell activation and plays a key role in maintaining immunological self-tolerance mediated by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice suggest its involvement in CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.