Thermo Fisher Scientific AMPK alpha 2 Monoclonal Antibody (5B4G1)
AMPK alpha 2 단백질을 표적하는 mouse monoclonal 항체로, Western blot, IHC, ICC, Flow cytometry, ELISA에 사용 가능. 인간 단백질에 반응하며, Protein G로 정제된 액상 형태. 에너지 대사 연구 및 AMPK 신호전달 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| Flow Cytometry (Flow) | 1:200–1:400 | |
| ELISA | 1:10,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B4G1 |
| Immunogen | Purified recombinant fragment of human PRKAA2 (AA: 453–552) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3091261 |
Target Information
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit and non-catalytic beta and gamma subunits. It functions as an energy-sensing enzyme that monitors cellular energy status. Upon activation by metabolic stress, AMPK phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and HMG-CoA reductase (HMGCR), enzymes involved in fatty acid and cholesterol biosynthesis. Studies in mice suggest that this subunit regulates insulin sensitivity and maintains myocardial energy homeostasis during ischemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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