Thermo Fisher Scientific DPH2 Monoclonal Antibody (5B10)
DPH2 단백질을 인식하는 Mouse monoclonal antibody (Clone 5B10). Western blot, ICC/IF, ELISA에 적합하며 Human과 Rat 시료에서 반응. Affinity chromatography로 정제된 액상형, PBS buffer 보존, -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.3 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B10 |
| Immunogen | DPH2 (NP_001375, 125–235 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
RQRSVALELC VKAFEAQNPD PKAPVVLLSE PACAHALEAL ATLLRPRYLD LLVSSPAFPQ PVGSLSPEPM PLERFGRRFP LAPGRRLEEY GAFYVGGSKA SPDPDLDPDL S
Target Information
This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis.
Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation.
Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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