Invitrogen™

TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F` Chemically Competent E. coli

TOPO™ TA Cloning™ Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1).자세히 알아보기

TOPO™ TA Cloning™ Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR™-TOPO™ Vectors include:

• 3´-T overhangs for direct ligation of Taq_-amplified PCR products
• Choice of T7 (pCR™2.1-TOPO™) or T7 and SP6 (pCR™II-TOPO™) promoters for _in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
• _Eco_R I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and ampicillin resistance genes for your choice of selection in _E. coli
_• Easy blue/white colony screening for selection of recombinants

TOPO™ TA Cloning™ Kits are also available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis.

사양

박테리아 또는 효모 균주TOP10F´

세포 유형Chemically Competent

클로닝 방법TOPO™-TA

형식Kit

제품라인One Shot™

제품 유형Cloning Kit

프로모터T7, SP6

수량25 Reactions

벡터TOPO-TA Cloning Vectors

Unit SizeEach