Thermo Fisher Scientific PNK Polyclonal Antibody
PNK 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot과 IHC에 적합합니다. A549, HeLa, HepG2 세포에 대한 양성 대조로 권장되며, 인간 PNK 단백질의 DNA 복구 과정 연구에 유용합니다. 액상 형태로 제공되며 4°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 78–521 of Human PNK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546329 |
Product Specific Information
- Recommended positive controls: A549, HeLa, HepG2
- Predicted reactivity: Mouse (82%), Rat (81%)
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Anti-human Polynucleotide Kinase (PNK) Polyclonal Antibody recognizes polynucleotide kinase (PNK), a 57 kDa nuclear enzyme involved in DNA strand repair processes during replication and recombination or in response to DNA damage.
PNK targets DNA 5'-hydroxy and 3'-phosphate termini for phosphorylation and dephosphorylation.
During strand breakage in human cells, PNK associates with nuclear proteins to prepare DNA termini for the enzymatic actions of DNA polymerase-beta and DNA ligase III.
PNK is a key component in maintaining the genetic stability of human cells.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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