Thermo Fisher Scientific ATP6V1B1 Monoclonal Antibody (OTI2D2), TrueMAB

Applications

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Vacuolar-type H⁺-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles.
It consists of a peripheral V1 domain (ATP hydrolysis) and an integral V0 domain (proton translocation).
The V1 domain comprises nine subunits (A–H), and the V0 domain comprises five subunits (a, d, c, c′, and c″).
V-ATPase likely operates via a rotary mechanism similar to F-ATPase.
The V1 B subunit exists as two isoforms:

• B1 isoform: Functions in proton secretion in the inner ear, maintaining proper endolymph pH and auditory function.
• B2 isoform: Expressed in kidney and osteoclasts.

Mutations in the B1 isoform gene (chromosome 2cen-q13) cause distal renal tubular acidosis associated with sensorineural deafness.

For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.