Thermo Fisher Scientific EGFR Recombinant Rabbit Monoclonal Antibody (30H45L48), Alexa Fluor 488
EGFR 단백질을 특이적으로 인식하는 Alexa Fluor 488 결합 토끼 단클론 항체. 세포 내 EGFR 발현 분석에 적합하며, 높은 특이성과 재현성을 제공. 인간 반응성, Expi293 발현 시스템 기반, 단백질 A 정제, 액상 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 30H45L48 |
| Immunogen | Proprietary |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2662332 |
Additional Formats
Product Specific Information
This antibody is predicted to react with canine, mouse, and rat based on sequence homology.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression of EGFR is associated with numerous cancers, making it a key target for both biological and small-molecule therapeutics.
EGFR is encoded by the EGFR gene on human chromosome 7 and belongs to the HER/ERbB family of receptor tyrosine kinases, which includes ERbB2, ERbB3, and ERbB4. Upon ligand binding (e.g., EGF, TGF-α), EGFR dimerizes and undergoes autophosphorylation, activating downstream signaling pathways.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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