Thermo Fisher Scientific Human TGF-alpha, Animal-Free Recombinant Protein, PeproTech
인체 유래 TGF-alpha 재조합 단백질로, EGF 수용체를 통해 세포 증식을 유도합니다. 무동물 성분으로 제조되었으며, ≥98% 순도와 낮은 엔도톡신 수준을 보장합니다. Balb/c 3T3 세포 증식 활성 확인. 실온 배송, -20°C 보관.
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Applications
Functional Assay (Functional)
- Assay-dependent
In vitro Assay (IV)
- Refer to 6 related publications
Miscellaneous PubMed (Misc)
- Refer to 2 related publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published Species | Horse, Human, Rat |
| Expression System | E. coli |
| Amino Acid Sequence | VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA |
| Molecular Weight | 5.5 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 98% (SDS-PAGE, HPLC) |
| Endotoxin Concentration | < 0.1 EU/µg |
| Activity | Stimulates proliferation of mouse Balb/c 3T3 cells. ED50 ≤ 0.2 ng/ml (≥ 5 × 10⁶ units/mg). |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Recombinant Human TGF-alpha is a 50 amino acid polypeptide (5.5 kDa) that shares approximately 40% sequence homology with EGF, including six conserved cysteine residues forming three intramolecular disulfide bonds.
This product is shipped at ambient temperature. For storage, handling, and reconstitution information, please refer to the lot-specific Certificate of Analysis.
Target Information
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor and stimulates the proliferation of a wide range of epidermal and epithelial cells.
It is produced by monocytes, keratinocytes, and various tumor cells, and induces anchorage-independent transformation in cultured cells.
Human, murine, and rat TGF-alpha are cross-species reactive. TGF-alpha (aa 50) binds to EGFR, activates tyrosine phosphorylation of the receptor, and promotes cell proliferation.
It also plays a role in tumor initiation by inducing a reversible transformed phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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