Thermo Fisher Scientific APE1 Polyclonal Antibody
Thermo Fisher Scientific의 APE1 Polyclonal Antibody는 인간 APE1 단백질을 인식하는 고품질 rabbit IgG 항체입니다. Western blot과 IHC(P)에서 검증되었으며, DNA 손상 복구 및 산화 신호 조절 연구에 적합합니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptides corresponding to amino acids 36–52 and 216–232 of human APE1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1956163 |
Product Specific Information
PA1-29465 detects APE from human samples.
Target Information
Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional enzyme involved in DNA repair and redox regulation of various transcription factors such as Fos, Jun, NF-κB, Myb, HIF-1α, CREB, Pax, and p53.
APE repairs abasic sites in DNA caused by spontaneous loss, oxidative damage, chemotherapy, ionizing radiation, UV irradiation, and oxidizing agents.
It shows differential expression across tissues and developmental stages and has diverse subcellular localization, suggesting interactions with multiple cellular proteins.
Regulation of APE by phosphorylation is mediated by casein kinase II.
APE levels increase upon reoxygenation of hypoxic cells and in some malignant tissues, while decreased expression is associated with apoptosis induction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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